Light microscopical and ultrastructural characterization of goat preantral follicles

In vitro culture of goat preantral follicles

Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...

Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles

The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter ...

Pneumocystis carinii pneumonia: a light microscopical and ultrastructural study.

A case of Pneumocystis carinii pneumonia developing a patient with treated diffuse lymphocytic lymphoma is described. The electron microscopic features and life cycle of the organism are illustrated. The patient died twenty-four hours after the biopsy had been taken. Septrin appeared to have affected the trophozoite stage. Pneumocystis carinii appears to induce interstitial pulmonary oedema and...

Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro1

Lima-Verde I.B., Matos M.H.T., Celestino J.J.H., Rossetto R., Name K.P.O., Báo S.N., Campello C.C. & Figueiredo J.R. 2012. Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro. Pesquisa Veterinária Brasileira 32(4):361-367. Programa de Pós-Graduação em Ciências Veterinárias, Laboratório de Manipulação de Oócitos e Folículo...

Light microscopical diagnosis of rabies